Journal: Veterinary Research
Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein
doi: 10.1186/s13567-025-01695-6
Figure Lengend Snippet: Specific antibodies and lymphocyte proliferative activity in piglets . A , B Dynamics of specific IgG and IgA in serum. C , D Dynamics of local specific mucosal IgA antibodies in saliva and nasal secretions, respectively. E Specific mucosal IgA antibody titres in the small intestine contents of piglets post-challenge. F Virus-neutralizing antibody titre in the serum of piglets in each group. G – I Stimulation indices of PBMCs and lymphocytes in PPs and MLNs at 14 days after challenge of piglets.
Article Snippet: The lymphocytes were isolated via density gradient centrifugation via peripheral blood lymphocyte isolation solution (Solarbio) and suspended in RPMI 1640 medium (Wisent) supplemented with 10% FBS (Wisent), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Solarbio).
Techniques: Activity Assay, Virus