human peripheral blood lymphocyte isolation solution (Beijing Solarbio Science)
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Human Peripheral Blood Lymphocyte Isolation Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 123 article reviews
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Isolation:Article Title: Identification, immunological function, and antimicrobial mechanisms of CXCL12 in turbot (Scophthalmus maximus). Article Snippet: The chemokine CXCL12, as a pivotal immune regulator, plays a crucial role in teleost fish against bacterial infection, but current research on its specific mechanisms remains scarce.. This study systematically identified two copies of the CXCL12 in turbot (Scophthalmus maximus) (SmCXCL12a and SmCXCL12b), investigated their expression patterns, and revealed their immune functions and antibacterial mechanisms.. Firstly, phylogenetic and syntenic analyses indicated that the SmCXCL12a/b were relatively conserved and exhibited high homology with orthologs in other teleost fish, including Japanese flounder (Paralichthys olivaceus). Article Title: Functional characterization of two CCL20 chemokines in black rockfish (Sebastes schlegelii): dual roles in immune cell recruitment and direct antimicrobial defense. Article Snippet: Using a heparinized syringe (Sigma-Aldrich), blood was collected from the caudal vein of recently euthanized black rockfish and subsequently diluted tenfold with Leibovitz (L-15) medium (Life Technologies), which contained 100 IU/mL of penicillin, 100 mg/mL of streptomycin (both from Life Technologies), and 5 % fetal calf serum (FCS; Life Technologies). .. Peripheral blood leukocytes were isolated with Article Title: Characterization, immune response and antibacterial mechanism of CXCL12 in black rockfish (Sebastes schlegelii). Article Snippet: Black rockfish (Sebastes schlegelii) is one of the most important mariculture fish in the Western North Pacific.. Although the crucial roles of CXCL12 in immune responses against bacterial infection are well-known, the specific mechanisms underlying its involvement in fish immunity remain poorly understood.. In this study, we systematically characterized CXCL12a and CXCL12b in Sebastes schlegelii (SsCXCL12a and SsCXCL12b), Firstly, SsCXCL12a/b were ubiquitously expressed across all seven tissues. Article Title: KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axis. Article Snippet: .. PBMCs were isolated with Article Title: Identification and immune function of the teleost-specific chemokine CCL39 in black rockfish ( Sebastes schlegelii ) Article Snippet: .. Following the instructions, peripheral blood leukocytes were isolated with Article Title: KRAS mutation promotes immune escape of lung adenocarcinoma via ZNF24/SLC7A5/PD-L1 axis Article Snippet: .. PBMCs were isolated with Concentration Assay:Article Title: Identification, immunological function, and antimicrobial mechanisms of CXCL12 in turbot (Scophthalmus maximus). Article Snippet: The chemokine CXCL12, as a pivotal immune regulator, plays a crucial role in teleost fish against bacterial infection, but current research on its specific mechanisms remains scarce.. This study systematically identified two copies of the CXCL12 in turbot (Scophthalmus maximus) (SmCXCL12a and SmCXCL12b), investigated their expression patterns, and revealed their immune functions and antibacterial mechanisms.. Firstly, phylogenetic and syntenic analyses indicated that the SmCXCL12a/b were relatively conserved and exhibited high homology with orthologs in other teleost fish, including Japanese flounder (Paralichthys olivaceus). Centrifugation:Article Title: Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential. Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were obtained by density layer centrifugation using |
